Russian Journal of Parasitology
UDK 619:616.995.132-079.4
DOI:10.12737/16654
Article history:
Received 24.09.2015
Accepted 24.11.2015
Link t full russian text
Download PDF format
.
Nematodes of the genus Setaria viborg, 1795 (Filariina setaridae), the parasites of the animals of uzbekistan morphological and molecular approaches to their differentiation
Azimov D.A. 1, Saparov K.A. 2, Akramova F.D. 1, Kuchboyev A.E. 1, Gaipova M.E. 3, Golovanov V.I. 1, Shakarbayev U.A. 1
1 Institute of Gene Pool of Plants and Animals, Academy of Sciences of the Republic of Uzbekistan 232 Bogishamol Str., Tashkent 100053, Uzbekistan E-mail: ushakarbaev@mail.ru
2Tashkent State Pedagogical University, Uzbekistan
3Tashkent State Agrarian University, Uzbekistan
Abstract
Objective of research: The purpose of the research is to study the species diversity of nematodes of the genus Setaria, the character of their distribution among the animals of Uzbekistan and morphologic and genetic approaches to their differentiation.
Material and methods. The mature forms of nematodes of the genus Setaria were studied with the use of the light microscopy method. The morphologic and molecular genetic testing of the species S. equina and S. labiatopapillosa was carried out with the use of common methods. Specimens of Setaria were taken from horses and cattle for the experiment.
Results and discussion: The mammals of Uzbekistan were recorded to be infected with 5 species of Setaria, which inhabited the abdominal cavity of the animals. The species under study (S. equina и S. labiatopapillosa) demonstrated almost the same morphological character; the limits of the morphological character’s variability almost coincided, except for the size of the spicules. The molecular genetic methods proved to be the most reliable methods of differentiation.
Key words: Setaria, nematodes, molecular biology, rDNA, ITS, Uzbekistan.
References
1. Arginskiy N. I. Nacekomie I kleshi, vredyashie sel’ckoxozyaystvennim jivotnim [Insects and ticks damaging agricultural animals]. Moscow: Sel’khozizdat, 1962. – pp. 74–91.
2. Кабилов Т. К. Gel’minti pozvonochnikh jivotnikh Uzbekistana, razvivayushiescz s uchastiem nasekomikh [Helminths in the vertebrates of Uzbekistan developing with the participation of insects]. – Tashkent: Fan, Uzb. SSR, 1983. –128 p.
3. Kuznetsov D. N. Rezul'taty sravnitel'nogo izucheniya speysernykh uchastkov ribosomal'noy DNK Telodorsagia circumcincta i T. trifurcata (Nematoda: Ostertaginae) [Comparative study of spacer rDNA domains of Telodorsagia circumcincta and T.trifurcata (Nematoda: Ostertaginae)]. // Rossiyskiy parazitologicheskiy zhurnal [Russian Journal of Parasitology]. Moscow, 2009, no 2. pp. 16–23. (In Russian).
4. Kuchboev A. E., Abramatov M. B., Khalilov I. M. i dr. Sravnitel'noe izuchenie vtorogo vnutrennego speysera (ITS2) ribosomal'noy DNK vidov Haemonchus contortus i H. placei (Nematoda: Trichostrongylidae) [Comparative study of the second internal transcribed spicer (ITS2) of ribosomal DNA of species Haemonchus contortus and H. placei (Nematoda: Trichostrongylidae)]. // Uzbekskiy biologicheskiy zhurnal [Uzbek Biological Journal]. Tashkent, 2012. no 1. pp. 38–42. (In Russian).
5. Saparov K. A., Golovanov V. I., Akramova F. D., i dr. Ekologo-faunisticheskiy analiz nematod podotryada Filariata – parazitov mlekopitayushchikh Uzbekistana [The ecologic and faunistic analysis of nematodes of the suborder Filariata, the parasites of the mammals of Uzbekistan]. // Rossiyskiy parazitologicheskiy zhurnal. Mockow, 2012. – № 4. – S. 29–37. (In Russian).
6. Saparov K. A., Akramova F. D., Shakarboev E. B. i dr. Fauna i biologicheskie osobennosti lichinok nematod, razvivayushchikhsya s uchastiem krovososushchikh nasekomykh [The fauna and biological characteristics of nematode larvae developing with the participation of blood sucking insects]. // Doklady AN RUz. Tashkent, 2013. – № 2. – S. 81–83. (In Russian).
7. Skrjabin K. I. Metody polnykh gel'mintologicheskikh vskrytiy pozvonochnykh, vklyuchaya cheloveka [The methods of complete helminthological dissection of vertebrates, including the human being]. M., L.: Izd. MGU, 1928. – 45 s. (In Russian).
8. Sonin M. D. Osnovy nematodologii. Filyariaty zhivotnykh i cheloveka i vyzyvaemye imi zabolevaniya [The principles of nematology. Nematodes of the suborder Filariata in animals and the human being and the diseases they cause]. – M.: Nauka, 1977. T. 28. – 220 s. (In Russian).
9. Sultanov M. A., Azimov D. A., Gekhtin V. I., Muminov P. A. Gel'minty domashnikh mlekopitayushchikh Uzbekistana [The helminthes of the domestic mammals of Uzbekistan]. – Tashkent, 1975. – 184 s. (In Russian).
10. Anderson R. K. Nematode parasites of vertebrates: their development and transmission. – New York: CAB International, 2000. – 650 p.
11. Casiraghi M., Bain O., Guerrero R. et al. Mapping the presence of Wolbachia pipentis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution// Int. J. Parasitol. 34, 2004. – P. 191–203.
12. Dallas J. F., Irvine J., Halvorsen O. DNA evidence that Marshallagia marshalli Ransom, 1907 and M. ocidentalis Ransom, 1907 (Nematoda: Ostertagiinae) from Svalbard reindeer are conspecific. // Systematic Parasitology, 2001. № 50. – R. 101–103.
13. Jayasinghe D. R., Wijesundera W. S. S. Differentiation of Setaria digitata and Setaria labiatopapillosa using molecular markers. // Vet. J., 2003. –165, – P.136–142.
14. Kuchboev A. E., Krucken J., Ruziev B. H., von Samson–Himmelstjerna G. Molecular phylogeny and diagnosis of species of the family Protostrongylidae from caprine hosts in Uzbekistan. // Parasitology Research 2015, 114 (4). – P 1355–1364.
15. Yatawara J., Wickramasinghe S., Nagataki M. et al. Molecular characterization and phylogenetic analisus of Setaria digitata of Sri Lanka based on COI and 12S rDNA genes. // Vet. Parasitology, 2007.–148.–P.161–165.
© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)