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Received 03.06.2014
Accepted 14.01.2015
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Biochemical changes in cattle body at fasciolosis
I. D. Shelyakin, S. N. Semyonov, O. M. Marmurova
Voronezh State Agricultural University named after Emperor Peter the Great 394087, Voronezh, 1 Michurin st., e-mail: ramon_ss@mail.ru
Abstract
Functioning of a number of transamination enzymes and alkaline phosphatase as well as some values of carbohydrate and lipid metabolism on background of protein in cattle blood at fasciolosis are studied. Total blood protein was determined by Loury method, alkaline phosphatase activity – by colorimetric method based on the hydrolysis of n-nitrophenol phosphate. The glutamate transferase activity was estimated by L.J. Olsen method, transaminase activity – by Reitman-Frankel method. Cholesterol levels were measured according to Liebermann–Burchard reaction, the pyruvate content was determined using the diphenylhydrazine method, and lactate – according to reaction with paraoxydiphenyl. The functional status of blood at fasciolosis is marked by the decrease in total protein and pyruvate with the increase of activity of glutamate transferase, alkaline phosphatase, lactate and cholesterol.
Keywords: cattle, fasciolosis, enzymes, total protein, carbohydrate metabol-ism, lipid metabolism.
References
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3. Lakin G. F. Biometrija [Biometry]. Moscow: Vysshaja shkola, 1980.
© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)