Evaluation of embryotropic properties of the anthelmintic Mitranox

Russian Journal of Parasitology
DOI: 10.12737/11779
Article history:
Received 26.02.2015
Accepted 24.03.2015
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Evaluation of embryotropic properties of the anthelmintic Mitranox


Kozlov S.A., Musaev M.B.
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin, 117218 Russia,
28 B. Cheremushkinskaya st., e-mail: musaev@vniigis.ru



Abstract


Objective of research: to estimate the potential embryotropic properties of the anthelmintic Mitranox.
Materials and methods: the experiments were conducted on 70 white outbreed pregnant rats divided into one control and 6 experimental groups (10 animals in each). The preparation was given orally to pregnant female rats of the 1st , 2nd and 3rd experimental group in a form of 1% starch suspension at the therapeutic dose 100 mg/kg, and to the rats of the 4th , 5th and 6th group at a triple dose 300 mg/kg in the critical periods of embryogenesis (1–6, 7–14 and 15–19 days of pregnancy).

Pregnant female rats of the control group received a 1% starch suspension. On the 20th day of embryogenesis all pregnant rats were killed by decapitation, and the embryos were examined according to Methodical Recommendations of the State Pharmacological Committee «Guidelines for experimental (preclinical) studies of new pharmaceutical substances», «Methodological guidelines for the hygienic assessment of new pesticides» and the Rules of Laboratory Practice in the Russian Federation.
Results: No pathological changes in the internal organs have been found. No malformation signs and other fetal and placenta abnormalities have been detected. The preparation has no embryotoxic effect because there was no difference in fetal mass and size between the control and experimental groups.
The pre- and post implantation death of rats and the total fetal mortality in experimental groups did not differ from the data of the control group.
No teratogenic effects of the drug were determined when the measurements of the average size of rudiments of embryonic skeleton were carried out.
Keywords: anthelmintic, Mitranox, embryotoxicity, teratogenicity, pre- and post implantation death, fetal mortality, malformations.



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© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)