Russian Journal of Parasitology
DOI:10.12737/13273
Article history:
Received 03.12.2014
Accepted 21.03.2015
Link to full russian text
Download PDF format
GENOTOXIC AND CYTOTOXIC EFFECTS IN HOST CELLS AT TAENIIDOSIS
Bekish V. Ya. , Zorina V. V.
Vitebsk State Medical University, Belarus
210023, Belarus, Vitebsk, 27 Frunze prsp., е-mail: bekishvl@tut.by
Abstract
Objective of research: The pork and beef tapeworm infestation is characterized by the increase of single-strand breaks, alkali-labile sites in nuclear DNA of bone marrow cells of golden hamsters. A triple subdermal sensibilisation with protein somatic products from the tissue of Taenia solium or Taeniarinchus saginatus is accompanied by a genotoxic effect in somatic bone marrow cells and generative cells in mouse seminal vesicles. It is manifested by increased single-strand breaks and alkali-labile sites in a nuclear DNA. The increase of damage of nuclear DNA depends on the dose of protein somatic products from the tissue of T. saginatus, it authentically grows up when a higher dose is used. Protein somatic products from the tissue of T. solium or T. saginatus at subdermal sensibilisation have a cytotoxic effect manifested in growth of apoptotic cells in bone marrow and seminal vesicles.
Materials and methods. To study the genotoxic and cytotoxic effects in genome of mammals at taenia infection and at triple subdermal sensibilisation with protein somatic products from the tissue of T. solium or T. saginatus the alkaline single cell gel electrophoresis by Singh et al., modified by Hellman et al. (comet) assay is used for investigation of bone marrow and seminal vesicles.
Results and discussion. The values of the alkaline single cell gel electrophoresis (comet) assay of bone marrow and seminal vesicles at T. solium or T. saginatus infection in golden hamsters were determined on 45th day of infestation.
Keywords: Taenia solium, T. saginatus, gel electrophoresis, genome damage, genotoxic and cytotoxic effects, golden hamster.
References
1. Astafyev B. A., Yarotsky L. S., Lebedeva M. N. Eksperimental’nye modeli parazitozov v biologii i meditsine [Experimental models of parasite diseases in biology and medicine]. Moscow, Nauka, 1989. 279 p.
2. Bekish V. Ya. Mutagenic activity of antigens from ascaris tissue. Zdravoohranenie [Health Care], 1999, no. 6, pp. 17–19. (In Russian)
3. Bekish V. Ya., Bekish O. Ya. Sostoyanie genoma hozyaina pri gel’mintozah [Status of host genome at helminthiasis]. Vitebsk, VGMU, 2004. 218 p.
4. Morozova N. A., Baryshnikova T. A. Determination of urinary protein by the biuret reaction. Laboratornoe delo [Laboratory work], 1991, no. 2, pp. 23–25. (In Russian)
5. Dyrnev A. D. Primenenie metoda shchelochnogo gel’-elektroforeza izolirovannyh kletok dlya otsenki genotoksicheskih svoystv prirodnyh i sinteticheskih soedineniy. Metod. rekomendatsii RAMN i RASN [Application of the alkaline single cell gel electrophoresis assay for estimation of genotoxic properties of natural and synthetic compounds]. Moscow, 2006. 27 p.
6. Hellman B., Vaghef H., Friis L., Edling C. Alkaline single cell gel electrophoresis of DNA fragments in biomonitoring for genotoxicity: an introductory study on healthy human volunteers. Int. Arch. Occup. Environ. Health, 1997, vol. 69, pp.185–192.
7. Herrera L. A. et al. Immune response impairment, genotoxicity and morphological transformation induced by Taenia solium metacestode. Mutat. Res., 1994, vol. 305, pp. 223–228.
8. Montero R., Ostrowsky P. Genotoxic activity of Praziquantel. Rev. in Mutat. Res., 1997, vol. 387, pp. 123–139.
9. Herrera L. A. et al. Possible association between Taenia solium cysticercosis and cancer: increased frequency of DNA damage in peripheral lymphocytes from neurocysticercosis patients. Trans. R. Soc. Trop. Med. Hyg., 2000, vol. 94, no. 1, pp. 61–65.
10. Flisser A. et al. Praziquantel treatment of brain and muscle porcine Taenia solium cysticercosis. Parasitol. Res., 1990, vol. 76, pp. 640–642.
11. Serrano–Garcia L., Montero–Montoya R. Micronuclei and chromatid buds are the result of related genotoxic events. Mutat. Res. Envir. and Mol. Mutagen, 2001, vol. 38, pp. 38–45.
12. Singh N., McCoy M., Tice R., Schneider E. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Research, 1988, vol. 175, pp. 184–191.
© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI) and the Agreement of 12.06.2014 (CABI.org / Human Sciences section).