Russian Journal of Parasitology
DOI:10.12737/13278
Article history:
Received 02.06.2015
Accepted 02.09.2015
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ELABORATION OF NEW METHODS FOR DECONTAMINATION OF FISH AND FISH PRODUCTS FROM METACERCARIAE OF Opisthorchis felineus Rivolta, 1884
Pelgunov A. N.
Center of Parasitology, Institute of Ecology and Evolution RAS,
119071, Moscow, Russia, 33 Leninsky prosp., e-mail: apelgunov@list.ru
Abstract
Objective of research: elaboration of effective and energy-efficiency methods for the decontamination of fishes of the larvae of Opisthorchis both in industrial and domestic environment.
Materials and methods: In the experiment we used orfes (nerflings) spontaneously infected with Opisthorchis felineus metacercariae. Vacuum treatment, electrodynamic shock vibration, ultrasonic irradiation, and microwave radiation were used for treatment of fish infected with opisthorchids. O. felineus metacercariae were identified by artificial digestion method. The viability of metacercariae was determined according to the preservation of morphological structure of metacercariae, their mobility, excystation of larvae using the trypsin-bile salt excystation method (experimental infestation of golden hamster and white outbreed mice).
Results and discussion: In was determined that O. felineus metacercariae are resistant to vacuum, electrodynamic shock vibration and ultrasound mostly due to the structure of their capsule.
Even when using the microwave we can observe the total decontamination of fish of Opisthorchis metacercariae.
Keywords: opisthorchiasis, microwave radiation, vacuum, electrodynamic shock vibration, ultrasonic irradiation, fish.
References
1. Beer S. A. Biologija vozbuditelya opistorkhoza [Biology of the agent of opisthorchiasis]. Moscow, KMK, 2005. 336 p.
2. Bulanova K. Ya., Solov’eva N. G., Lobanok L. M. Biological and medical aspects of the effect of electromagnetic fields. Izvestiya NAN Belorusi. Ser. Biologiya [Bulletin of National Academy of Sciences Belarus, Ser. Biology], 2003, no. 4, 108 p.
3. Glazkov G. А., Klimshin А. А., Krivenko A. I. Method of sanitary-helminthological examination of fish for the presence of larvae of opisthorchids and determining their viability. Gigiena i sanitariya [Hygiene and sanitation], 1979, no. 8, pp. 53-55.
4. Kondrat'ev V. N. Photochemical reactions and reactions in electrical discharge. Kinetika khimicheskikh reaktsiy [Kinetics of chemical reactions]. Moscow, Publ. of the USSR Academy of Sciences, 1958, pp. 79-127.
5. Pauling L. Priroda khimicheskoj svyazi [The nature of the chemical bond]. Moscow-Leningrad, 1947. 440 p.
6. Pel'gunov А. N., Ryabov I. N., Filippova А. Yu. The use of microwave ovens for the prevention of opisthorchiasis. Meditsinskaya parazitologiya i parazitarnye bolezni [Medical parasitology and parasitic diseases], 2005, no. 3, pp. 42-45.
7. Profilaktika parazitarnykh boleznej na territorii Rossijskoj Federatsii (Sanitarnye pravila i normy SanPiN 3,2,569-96) [Prevention of parasitic diseases on the territory of the Russian Federation (Sanitary rules and norms SanPiN 3,2,569-96]. Moscow, Information and publishing centre of the Ministry of Health, 1997. 168 p.
8. Rogov I. А., Аdamenko V. Ya. Sovremennye metody i oborudovaniye dlya sverkhvysokochastotnoy obrabotki pishhevykh produktov v promyshlennosti (obzor) [Modern methods and the equipment for ultra-high-frequency processing of foodstuff in the industries (Review)]. Moscow, Central Food Technological Research Institute, 1971. 54 p.
© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI) and the Agreement of 12.06.2014 (CABI.org / Human Sciences section).