Russian Journal of Parasitology
Article history:
UDK 619:616.995.132:1-07
DOI: 10.12737/16665
Received 01.08.2015
Accepted 24.11.2015
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Method for releasing of larvae toxocara canis from liver and lung parenchyma of carnivores
Panova O.A.1, Glamazdin I.G.1, Shibitov S.K.2
1Moscow State University of Food Production, 125080 Moscow, 11 Volokolamskoye shossee ,e-mail: Glamazdin@yandex.ru
2All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin, 117218 Russia, 28 B. Cheremushkinskaya St., Central Scientific and Methodical Veterinary Laboratory
Abstract
A method for releasing of larvae Toxocara canis from liver and lung parenchyma of carnivores for post-mortem diagnostics of toxocarosis under light invasion and in prepatent period has been suggested.
The received culture of larvae T. Canis can be used to study disease pathogenesis, conduct genetic research and obtain proteins with diagnostic and protecting properties.
A list of equipment, reagents and solutions for releasing of larvae T. canis is presented.
Course of work including preparation of liver and lungs samples, gastric juice, digestion of tissue samples, results estimation, concentration of larvae T. canis has been described. Samples of liver and lung parenchyma of mass 50 g are being obtained and grinded in a meat mincer. Samples are being digested within 50 min under the temperature 41-42 о С by permanent stirring.
After 10 minutes of settling the sediment is being poured into Petri dishes and investigated for presence of larvae T. canis and their mobility. For material concentration the sediment is being centrifuged 10 min at 5000 rpm.
Keywords: larvae , Toxocara canis, liver, lungs, carnivores, digestion, diagnostics.
References
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2. Zamazij T.N., Zdor O.A. Osobennosti jepidemiologii i klinicheskogo techenija toksokaroza v sovremennyh uslovijah // Mezhdunar. med. zhurnal. 2005. – № 1. – S. 133-135.
3. Solopov P.A. Immunofermentnyj metod diagnostiki toksokaroza sobak, serojepizootologicheskij monitoring i terapija: Dis. … kand. vet. nauk. - Rjazan', 2009. – 114 s.
4. Tumol'skaja N.I., Sergiev V.P., Lebedeva M.N. i dr. Toksokaroz. Klinika. Diagnostika. Lechenie. Profilaktika. – Novosibirsk, 2004. – 48 s.
© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)