Modified technique of prepare of total permanent preparations of parasitic flatworms

MODIFIED TECHNIQUE OF PREPARE OF TOTAL PERMANENT PREPARATIONS OF PARASITIC FLATWORMS

V.V. Kuklin

Modified method of prepare of permanent stained preparations by cestodes and trematodes is described. Using of 5 % solution of KOH and 2–3 % solution of HCl before painting by carmine of worms fixing by 70 % alcohol and supplementary fixation of worms by 70 % alcohol after painting are recommended.

Keywords: total preparation, Cestoda, Trematoda, carmin.


© 2016 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI) and the Agreement of 12.06.2014 (CABI.org / Human Sciences section).