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Changes of values obtained in Single Cell Gel Electrophoresis of host somatic cells at experimental opisthorchosis
D.K. Kuzhel
postgraduate
V.J. Bekish
doctor of medical sciences
V.V. Zorina
PhD in biological sciences
Vitebsk State Medical University
Belarus, Vitebsk, 210023, Frunze prosp. 27, e-mail: bekishvl@tut.by
On applying the method of alkaline Single Cell Gel Electrophoresis (SCGE) it has been established that metabolites of cat liver fluke maritas have a genotoxic influence on somatic cells of golden hamsters. Genotoxic influence is observed in blood cells of animals on the 7, 14, 21, 28, 35, 60, 90, 120 and 150-th days of invasion with the maximum 8,2 times expression on the 14-th day of invasion. The indicator «the comets tail moment» in bone marrow cells was 1,9–6,5 times higher than the control values with the maximum expression on the 21-st day of invasion. Maximum genotoxic 7,1 times effect in the liver was observed on the 14-th day of invasion. The level of apoptotic cells in blood, bone marrow and liver of animals in experimental opistorchosis increases due to cytotoxic effect of invasion. Cytotoxic effect of metabolites of cat liver fluke maritas is observed in blood on the 7, 14, 21, 28, 35, 60, 90, 120 and 150-th days with the maximum 8,3 times expression of these changes on the 21-st day. In bone marrow the maximum 10,5 times degree of cells apoptosis was observed on the 14 and 28-th days of invasion. The liver cells apoptosis in infected animals 2,3-6,2 times exceeded the control values with the maximum expression of these changes on 28-th day of observation.
Keywords: opistorchosis, method of «DNA-comet assay», golden hamsters, genotoxic and cytotoxic effects.