The method of allocation of Toxocara canis larvae from parenchyma of liver and lung of carnivores

Link t full russian text
Download PDF format


The method of allocation of Toxocara canis larvae
from parenchyma of liver and lung of carnivores

O. A. Panova
postgraduate
I.G. Glamazdin
doctor of veterinary sciences
Moscow State University of food productions
125080, Moscow, Volokolamskoye Highway, 11, e-mail: glamazdin@yandex.ru
S. K. Shibitov
Central scientific and methodical veterinary laboratory,

The method of allocation of Toxocara canis larvae from parenchyma of liver and lung of carnivorous animals for postmortem diagnostics of toxocarosis is offered at a weak infection and in prepatent period. The received culture of larvae of T. canis can be used for studying pathogenesis of the diseases, carrying out genetic researches and receiving proteins with diagnostic and protective properties. The list of the equipment, reactants and solutions for allocation of T. canis larvae is given. The work course which consists of preparation of tests of tissue of a liver and lungs, preparation of artificial gastric juice, digestion of tissues, an assessment of the results, concentration of T. canis larvae is described. For researches take samples of a parenchyma of lungs or a liver weighing 50 g, crush on a meat grinder. Samples digest to the current of 50 min. at a temperature of 41–42 °C. After 10 minute upholding a deposit merge in Petri's cups and investigate on existence of T. canis larvae and their mobility. For concentration of a material a deposit centrifuge during 10 min.

Keywords: larvae, Toxocara canis, liver, lungs, carnivorous, digestion, diagnostics.