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INFLUENCE OF ANTHELMINTIC DRUGS ON ACTIVITIES OF 5'-NUCLEOTIDASE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE)
E. A. Burenina Doctor of biological sciences
Institute of Biology and Soil Sciences, Far East Branch of RAS, Vladivostok, Russia 690022, Prsp.100-let Vladivostoku, 159, e-mail: Burenina@ibss.dvo.ru
Activities and properties of 5'-nucleotidase in the subcellular fractions of Bothriocephalus scorpii are studied with the use of adenosine monophosphate, inosine monophosphate and cytidine monophosphate as substrates. The high activity of 5'-nucleotidase was observed in the mitochondrial fraction. Dependence of the 5'-nucleotidase activity on the concentration of substrates and Mn2+ ions is investigated. Impact of various effectors and ions (sodium fluoride, EDTA, cysteine, K+, Na+, Zn2+, Ca2+, Ba2+, Cu2+, Mn2+) on enzyme activity is determined. Effect of 10 anthelmintic drugs on enzyme activity has been tested. The most effective drugs are bitionol and oxinide.
Keywords: 5'-nucleotidase, adenosine monophosphate, inosine monophosphate, cytidine monophosphate, mitochondria, cestoda, anthelmintic drugs.
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© 2016 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
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