Russian Journal of Parasitology, 2016, V.35, Iss.1
DOI: …………………
Received 12.01.2016
Accepted 19.02.2016
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Skvortsova F. K., Uspenskiy A.V. Diagnostics of trichinosis in the early stages of larval development. Russian Journal of Parasitology, 2016, V.35, Iss.1, pp.
DIAGNOSTICS OF TRICHINOSIS IN THE EARLY STAGES OF LARVAL DEVELOPMENT
Skvortsova F. K., Uspenskiy A.V.
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin, 117218 Russia, 28 B. Cheremushkinskaya St., e-mail: scvorcova@vniigis.ru, director@vniigis.ru
Abstract
The research objective – Confirmation of the diagnosis on trichinosis depends on many factors among which sampling of muscular tissue from the parts of hulk, a method of research and the used equipment defined most the invazirovannykh appears.
Materials and methods. The research material were samples of muscle tissue from experimentally T. spiralis infected rats. Was infected 18 white mongrel rats weighing 90-100g at a dose of 10 l/G. For the early diagnosis of trichinosis when the rats were killed with 6 to day 24 after infection. First investigated by the compressor method available and the most affected muscles: the diaphragm, masseter and chest muscles by increasing (x 25, 50, 100). Each term is made photomicrographs of larvae on the slices.
Then the samples of the hindlimb muscle mass of 50 g was investigated by automated method of peptonize apparatus Gastros. At the end of the cycle of operation of the apparatus when microscopy was considered the quantity of larvae and their morphological development. Selected larvae 16 to 18 days of age have been infected mice (bioassay). After 35-36 days carcass mice were fully exposed to patalsu for the detection of Trichinella.
Results and discussion. When peptonize automated method muscle tissue of rats infected with larvae of T. spiralis, found that larvae aged from 10 to 15 days after infection by the method are not detected. Trichinae isolated from 16-day-old, identified by microscopy, despite the lack of size of the larvae. The massive selection of Trichinella begins with the 17th day after infection, when most of the larvae reaches invasionist.
Non-encapsulated trichinae 16 days of age become invasive, which showed successful infection of white mice with larvae that are highlighted after peptonize. According to the results of a biosample main mass of larvae becomes infective to 19-20 days after infection.
Thus, an automated method of peptonize muscle tissue easily diagnose the infection of animals with non-encapsulated larvae of Trichinella that are difficult or sometimes impossible in compressor research. Diagnosis of trichinosis by automated method is most effective when the larvae of invasionist.
Keywords: trichinosis, patolis apparatus Gastros, trichinoscopy, Trichinella spiralis, T. Pseudospiralis.
© 2015 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI) and the Agreement of 12.06.2014 (CABI.org / Human Sciences section).