Efficacy of the supramolecular complex of Fenbendazole against nematodiasis in sheep

Russian Journal of Parasitology, 2016, V.35, Iss.1
Received   01.10.2015 
Accepted  19.01.2016
Link t full russian text
Download PDF format


Efficacy of the supramolecular complex of Fenbendazole against nematodiasis in sheep

Varlamova A. I.1, Limova Yu. V.2, Sadov K. M.2, Sadova A. K.2, Belova E. E.1, Radionov A. V.1, Halikov S. S.3, Chistyachenko Yu. S.4, Dushkin A. V.4, Skira V. N.5, Arkhipov I. A.1
1All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin
117218, Moscow, 28 B. Cheremushkinskaya St., e-mail: arkhipov@vniigis.ru
2 Samara Research Veterinary Station, e-mail: samnivs@mail.ru
3 The A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, 28 Vaviov St., e-mail: salavatkhalikov@mail.ru
4  Institute of Solid State Chemistry and Mechanochemistry SB RAS,
630128, Novosibirsk, 28, Kutateladze St., e-mail: dushkin@solid.nse.ru
5 Federal Agency of Scientific Organizations of the Russian Federation
119334, Moscow, 32а Leninsky ave., e-mail: akvet@mail.ru


Objective of research:  to study the anthelmintic efficacy of the supramolecular complex of Fenbendazole used against different nematode species in sheep

 Materials and methods: Experiments were carried out on young sheep spontaneously infected with gastrointestinal strongylates (48 head), Dictyocaulus filaria (42 head), Strongyloides papillosus (21 head) и Trichocephalus ovis (24 head). In each helminthiasis, the supramolecular complex of Fenbendazole was given once orally to sheep from various groups at the dose of 3,0; 2,0 and 1,0 mg a.i./kg in comparison with the base preparation Fenbendazole at the doses of 1,0 and 3,0 mg/kg. Sheep which didn’t receive the drug served as controls.

The efficacy of drugs was evaluated before and 18 days after dehelmintization according to the results of coprolarvoscopic examination by flotation and G. Baermann methods. The registration of drug activity was performed using the «control test».

Results and discussion:  Anthelmintic efficacy was studied and a therapeutic dose for the supramolecular complex of Fenbendazole produced by chemical mechanical technology using the Drug Delivery System was determined. In gastrointestinal strongylatoses the supramolecular complex against D. filaria. at the doses of 3,0; 2,0 and 1,0 mg a.i./kg showed the efficacy of 100; 93,4 and 78% , respectively.   

The efficacy of supramolecular complex at the dose of 3,0 mg/kg against S. papillosus was 100 %, and against T. ovis - 98,3 % at 10–13% efficacy of the base preparation Fenbendazole  at the dose of 1,0 mg/kg. The therapeutic dose for the supramolecular complex at main nematodiasis in sheep was 3,0 mg a.i./kg.

Keywords: sheep, Strongylata, Dictyocaulus filaria, Strongyloides papillosus, Trichocephalus ovis, supramolecular complex, Fenbendazole, efficacy.


1.  Arhipov I. A. Antigel'mintiki: farmakologiya i primenenie [Anthelmintics: pharmacology and use]. M., 2009. 406 p. (in Russian)

2. Dushkin A. V., Suncov L. P., Halikov S. S. Mechanochemical technology for improving solubility of drugs.  Fundamental'nye issledovaniya [Fundamental research], 2013, no. 1 (Part 2), pp. 448–457.

3. Bossche Н., Rochette F., Horig С. Anthelmintic efficacy of fenbendazole. Vet. Rec., 1982, vol. 78, no. 3, pp. 876–877.

4.  Düwell D., Strasser H.  Wirkung von Fenbendazol bei parasitischen Krankheiten. Dtsch. Tiearztl. Wsch., 1978, vol. 85, no. 2, pp. 239–241.

5. Kalpana P., Manish S., Dinesh S.K., Surenda J.K. Solid dispersion: approaches, technology involved, unmet need & challenges. Drug Invent. Today, 2010, vol. 2, no. 7, pp. 349–357.

6. Krishnaian Y. S. R. Pharmaceutical technologies for enhancing oral bioavailability of poorly soluble drugs. J. Bioequival. Bioavailab., 2010, vol. 2, no. 2, pp. 28–36.

7. Shinde A. J. Solubilization of poorly soluble drugs: A Review. Pharma Infonet., 2007, 5(6), pp. 157–159.

8. The Biopharmaceutics classification system (BCS) guidance, available

at: http://www.fda.gov/AboutFDA/CentersOffices/CDER/ucml 28219.htm

© 2015 The Author(s). Published by All-Russian Scientific Research Institute of  Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin.
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)