Screening of new chemotherapeutic agents to combat fish diseases caused by parasitic Diplomaragnidae (Diplomonadida wenyon, 1926)

Russian Journal of Parasitology, 2016, V.35, Iss.1
DOI: …………………
Received 02.10.2014
Accepted 14.01.2016
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Gavrilin K. V.1, Bychkova L. I.1, Dmitriev S. N.1, Mamykin G. A.2
1 Moscow state University of technologies and management. K. G. Razumovsky (First Cossack University)
109004, Moscow, Zemlyanoy Val, 73, e-mail:
2 Centre for fish breeding and disease control of fishes (LLC "Rebbetzen")
123001, Moscow, Ermolaevsky pereulok, 18a, e-mail:



Objective of research. The purpose of the study – screening of new chemotherapeutic agents for the treatment of hexamita ornamental fish.

Materials and methods. The test drugs were performed on juveniles of the discus Symphysodon discus a body mass of 15 g, spontaneously infested hexamitidae (Diplomonadida). 35 discus of the infected individuals were divided into 7 groups of 5 copies of the Fish 1, 2, 3, 4, 5, and 6th groups were appointed in the composition of granulated feed, respectively pyrimethamine at a dose of 50 mg/kg albendazole – 50, fenbendazole – 50, mebendazole – 10, magnesium sulfate – 500 and metronidazole in a dose of 50 mg/kg for 5 days. Fish 7 the first group was control and received feed without the drug. The effectiveness was considered after 5 days after the last Dachi medicated feed. Fish were dissected, the intestines were isolated, which were released from the food pulp, homogenized, and were studied by optical microscopy at a magnification of 180 times.

Results and discussion. The number of examined in treated fish compared with the control decreased after the application of metronidazole by 95.5 %, mebendazole – 77.8, magnesium sulfate, which is 74.5, fenbendazole – by 56.6, albendazole – 50.5 and pyrimethamine – 24.4 %. Advanced for further testing are mebendazole, magnesium sulphate, fenbendazole and albendazole. The most effective was a basic drug – metronidazole. During the test degradation of fish were noted. Food activity was moderate at the level of 2% of ichthyomass. In fish treated with metronidazole, mebendazole and magnesium sulfate, have improved the condition.

Key words: ornamental fish, hexamita, treatment, metronidazole, mebendazole.

 © 2015 The Author(s). Published by All-Russian Scientific Research Institute of  Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin. 
This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI)) and the Agreemnt of 12/06/2014 (CABI org / Human Sciences section)