Nucleoside-diphosphatase of cestode bothriocephalus scorpii (cestoda: bothriocephalidae).

Link t full russian text
Download PDF format

Russian Journal of Parasitology, 2016, V.38, Iss.4
Received 10.02.2016
Accepted 28.11.2016



Burenina E. A.
Institute of Biology and Soil Sciences, Far East Branch of RAS
690022, г. Vladivostok, 159 Prosp.100-letiya Vladivostoka,
e-mail: burenina @



Objective of research: To study the activities and properties of nucleoside- diphosphatase (NDPase) in cestode Bothriocephalus scorpii.

Materials and methods:  Cestodes were homogenized with 10 vol. of extraction medium. NDPase was detected in mitochondria and microsomes with substrates (IDP, GDP, UDP). Inorganic phosphorus was determined by the method of Kochetov (1980). The effects of 10 anthelmintic drugs on the activity of NDPase were studied.

Results and discussion: It was found that the mitochondrial and microsomal fractions of cestodes B. scorpii have nucleoside diphosphatase activity.

The activity of nucleoside diphosphatase depends on substrates and Mg2+ ions. 

The impact of various effectors and ions (Ca2+, Mn2+, Zn2+) on enzyme activity was determined.

Effects of 10 anthelmintic drugs on activity of nucleoside diphosphatase were studied. The anthelmintics Bitionol and Trichlorophen have been proved effective.

Keywords: nucleoside-diphosphatase, inosine diphosphate, uridine diphosphate, guanosine diphosphate, cestode, mitochondria, microsomes, anthelmintic drugs.


1.      Bogitsh B. J., Krupa P. L. Schistosoma mansoni and Haematoloechus medioplexus: nucleosidediphosphatase location in tegument. Exptl. Parasitol., 1971, vol. 30, no. 3, pp. 418–425.

2.      Failer B. U., Braun N., Zimmermann H. Cloning, expression and functional characterization of a Ca2+- dependent endoplasmic reticulum nucleoside diphosphatase. J. Biol. Chem., 2002, vol. 277, pp. 36978–36986.

3.      Gounaris K., Selkirk M. E., Sadeghi S. J. A nucleotidase with unique catalytic properties is secreted by Trichinella spiralis. Mol. Biochem. Parasitol., 2004, vol. 136, no. 2, pp. 257–264.

4.      Kochetov G. A. Method for determination of organic phosphorus. Prakticheskoe rukovodstvo po enzimologii [Practical guide on enzymology] M., 1980, pp. 215–216.

5.    Kornish–Bowden A. Osnovy fermentativnoy kinetiki. [Principles of Enzyme Kinetics]. M., Mir, 1979.

6.      Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with the Folin  phenol reagent. J. Biol. Chem., 1951, vol. 193, no. 1, pp. 265–275.

7.      Moczon T. Histochemical studies on the enzymes of Hymenolepis diminuta. II Nonspecific and specific phosphatases in oncospheres and cysticercoids. Acta Parasitol. Pol., 1973, vol. 21, no. 1–10, pp. 99–106.

8.      Moczon T. Histochemical studies on the enzymes of Hymenolepis diminuta. IV. Nonspecific phosphatases in a mature parasite. Acta Parasitol. Pol., 1974, vol. 22, no. 22–34, pp. 323–329.

9.      Roy T. K. Histochemical studies on Raillietina (Raillietina) johri (Cestoda: Davaineidae). II. Nucleoside diphosphatase and thiamine pyrophosphatase. J. Helminthol., 1979, vol. 53, no. 3, pp. 261–263.

10. Roy T. K. Distribution, functional significance of phosphatase in the bovine amphistome Ceylonocotyle scoliocoelium. Ind. J.  Exp. Biol., 1980, vol. 18, no. 4, pp. 385–392.

11. Seniuta R. Cytochemical studies on some specific phosphatase activities in experimental trichinellosis of mice. Wiadon. Parasitol., 1975, vol. 21, No 4–5, pp. 683–688.

12. Strauss O., Graszynski K. Isolation of plasma membrane vesicles from the gill epithelium of the crayfish Orconectes limosus Rafinesque and properties of the Na+/H+ enchanger. Comp. Biochem. Physiol., 1992, vol. 102A, no. 3, pp. 519–526.

© 2016 The Author(s). Published by All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin. This is an open access article under the Agreement of 02.07.2014 (Russian Science Citation Index (RSCI) and the Agreement of 12.06.2014 ( Sciences section: