Received 15.03.2016
Accepted 28.11.2016
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Kochetkov P.P., Varlamova A.I., Abramov V.E., Misura N.S., Abramova E.V., Abramov S.B., Koshevarov N.I., Arkhipov I.A. Determination of fenbendazole and its metabolites in milk by the method of liquid chromatography coupled with tandem mass-spectrometry // Russian Journal of Parasitology, 2016, V.38, Iss.4, pp.
DETERMINATION OF FENBENDAZOLE AND ITS METABOLITES IN MILK BY THE METHOD OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRY
Kochetkov P.P.1,2, Varlamova A.I.1, Abramov V.E.1, Misura N.S.2, Abramova E.V.1,2, Abramov S.B.1,2, Koshevarov N.I.2, Arkhipov I.A.11 All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin, 117218, Russia, Moscow, 28 B. Cheremushkinskaya St., e-mail: varlamova@vniigis.ru
2 International Research Center for protection of human health, animals and environment., 117218, Russia, Moscow, 28 B. Cheremushkinskaya St., e-mail: cozos@mail.ru
Abstract
Objective of research: Development of methods for the determination of fenbendazole and its metabolites in milk by liquid chromatography coupled with tandem mass spectrometry.
Materials and methods: Fenbendazole was administered orally to five cows. Samples of milk were taken on 1, 3, 5 and 10 days of drug application. The research method includes a description of reagents, plates and equipment; mass-spectrometric conditions for analysis of fenbendazole and its metabolites; preparation of the equipment to operation; preparation of eluent solution; preparation of the chromatograph to analysis; determination of chromatographic parameters of standard drug samples; preparation of milk samples to analysis; establishment of parameters of extracts’ chromatography; procedure of calibration of fenbendazole and its metabolites in eluent.
Results and discussion: When studying the pharmacokinetics of fenbendazole and its metabolites (sulfone and sulfoxide) in milk, it was found that the maximal concentrations were determined 24 h after drug administration and were 22,6 ng/ml for fenbendazole sulfone, 34,0 ng/ml for fenbendazole sulfoxide and 19,7 ng/ml for fenbendazole. 10 days after treatment, the concentrations of fenbendazole and its metabolites in milk did not exceed permitted values.
Keywords: milk, fenbendazole, metabolite, sulfoxide, sulfone, pharmacokinetics, liquid chromatography, mass-spectrometry.
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