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Russian Journal of Parasitology, 2017, V.39, Iss.1
Received: 30.03.2016
Accepted: 10.03.2017
PROTECTIVE PROPERTIES OF ECHINOCOCCUS MULTILOCULARIS PROTOSCOLEX ANTIGENS IN COMBINATION WITH THE IMMUNOMODULATOR RONCOLEUKIN IN SECONDARY ALVEOLAR ECHINOCCOCOSIS
Berezhko V.K., Rudneva O.V., Sasikova M.P.
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin, 117218 Russia, 28 B. Cheremushkinskaya St., e-mail: berejko@vniigis.ru, olgaru79@mail.ru, marina_sasikova@mail.ru
Abstract
Objective of research: Estimation of protective effects of Echinococcus multiocularis protoscolex cell antigens in combination with the immunomodulator Roncoleukin in experimental alveolar echinococcosis.
Materials and methods: In this experiment we used cellular antigens - E. multilocularis protoscoleces cell metabolites cultivated in artificial substrate [Berezhko V.K. and co-authors, 2001], and immunomodulator Roncoleukin (pharmaceutical form of recombinant human interleukin-2 (rhIL-2). Experiments were conducted on 48 white outbreed mice with the mass of 18-20 g. divided into 4 equal groups. The first group of mice received Roncoleukin twice subcutaneously, with a ten-day interval at the dose of 180 МЕ in 0,2 ml of sterile 0,9% sodium chloride solution; the second group (according to the same schedule) - cell antigens at the dose of 60 mсg protein antigen per mouse and Roncoleukin at the same dose; the third (according to the same schedule) - cell antigens at the dose of 60 mсg protein antigen per mouse. The fourth group (controls) received 0,2 ml of sterile 0,9% sodium chloride solution per mouse.
After 20 days, all mice were infected with protoscoleces and acephalocysts of E. multilocularis at the dose of 750±50 ind./mouse. On the 90th day of invasion, the experimental mice were killed and underwent autopsy according to the «Rules for conducting works using experimental animals».
Results and discussion:
The results of the experiment showed that the protective effect in the first group receiving Roncoleukin was 58,3%; the third group (cell antigens of protoscoleces - 66,7%); the second group (complex preparation containing Roncoleukin and cell antigens)- 83,3%. Single larval cysts on liver parenchyma without embryonic elements were detected in 2 mice only of the group receiving a complex preparation. In the other two groups, parasitic cysts were registered in 5 and 4 mice with the mass 125,3±21,8 – 142,2±38,02 mg and the size 11,98±4,2 – 12,35±3,46 mm, respectively.
All mice from the control group were infected. Numerous larval cysts (size 15,64±1,46mm and mass 580,8±222,09 mg) with developing viable protoscoleces were found in abdomen and internal organs.
Keywords: Echinococcus multilocularis, protoscoleces, cell antigen, immunomodulator, Roncoleukin, alveolar echinococcosis.
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